ABSTRACT
Objective To explore the role of the Hedgehog singaling in the pathogenesis of esophageal cancer.Methods Tissue samples of normal esophageal mucosa,low-grade dysplasia,high-grade dysplasia and esophageal cancer were collected.Immunohistochemical stain,Western-blot and RT-PCR were employed to detect theexpression of Hedgehog protein and gene,methylation special PCR was used to detect the methylation status of Hedgehog gene.Results There was no difference in the expression of SHh protein among nornaml esophageal and low-grade dysplasia,high-grade dysplasia and esophageal cancer(LGD vs.N:t=1.96,P=0.67;HGD vs.EC:t=1.59,P=0.53).However,the expression of SHh protein in high-grade dysplasia and esophageal cancer was higher than that in normal esophageal mucosa and low-grade dysplasia(HGD vs.N:t=0.593,P=0.004;HGD vs.LGD:t=1.308,P=0.325;EC vs.N:t=0.292,P=0.000;EC vs.LGD:t=0.734,P=0.004).The expression of Hedgehog gene in esophageal cancer was higher than that in normal esophageal mucosa,low-grade dysplasia and high-grade dysplasia(EC vs.N:t=0.909,P=0.019,EC vs.LGD:t=0.398,P=0.007;EC vs.HGD:t=0.843,P=0.012).The gene methylation in esophageal cancer was lower than that in normal esophageal mucosa,low-grade dysplasia and high-grade dysplasia(EC vs.N:t=0.0340,P=0.000;EC vs.LGD:t=0.102,P=0.000;EC vs.HGD:t=0.367,P=0.018).Conclusion Hedgehog signaling may play a role in the pathogenesis and development of esophageal cancer,however,demethylation of Hedgehog gene may be one of the mechanism that cause the activity of oncogene in esophageal cancer.
ABSTRACT
Objective To explore the effect of aspirin combined with clopidogrel bisulfate in the treatment of patients with acute cerebral infarction.Methods 486 patients with acute cerebral infarction were selected and divided into the two groups in accordance with the random number table,243 cases in each group.The control group were given aspirin and conventional medical treatment.The observational group were given aspirin and clopidogrel bisulfate orally,system hemorrhage and TT,PT,APTT,FIB of both group were monitored.The efficacy was analyzed in accordance with the degree of neurological deficits score.Results The NIHSS of the observational group after treatment [(6.38 ± 1.19) points] was significantly lower than that of before [(15.04 ± 2.53) points] (P ＜ 0.05);The NIHSS of the control group after treatment [(9.45 ± 3.27) points] was significantly lower than before [(16.26 ± 2.71) points] (P ＜ 0.05);The NIHSS after treatment in the observational group [(6.38 ± 1.19) points] was significantly lower than that of the control group [(9.45 ± 3.27) points] (t =8.264,P ＜ 0.05).The total effective rate of the observational group was 94.24％,which was significantly higher than 81.48％ of the control group(x2 =11.485,P ＜ 0.05).No system hemorrhage was found in both groups.The coagulation index after treatment was higher than before but still in relatively safe range.There was no significant difference of coagulation index between observational group and control group (all P ＞ 0.05).Conclusion Using aspirin combined with clopidogrel bisulfate in acute cerebral infarction patients is safe and effective.